Objective To explore the effect of 3D laparoscopic assisted on the learning effect of students in clinical teaching. Methods A total of 56 students were divided into control group and observation group, each with 5 years of undergraduate internship and training doctors. The control group was taught by traditional teaching methods. The observation group was treated with 3D laparoscopic assisted teaching. The two groups were 120 minutes. Compare the two groups of students to master the knowledge of the situation and the evaluation of the two teaching methods. Results The teaching results and satisfaction degree of the 3D laparoscopic assisted teaching group were significantly better than those of the traditional teaching group (91.6±4.5) and (80.5±4.8), respectively. The difference was statistically significant (t=2.18, P<0.05). Conclusion Through advanced 3D laparoscopic assisted surgery, students can provide real, image, vivid, comprehensive disease related image information, improve students' interest in learning, but also make it easier for students to understand the clinical knowledge.